human normal cervical epithelial cells Search Results


human normal cervical epithelial cells hucec  (ATCC)
93
ATCC human normal cervical epithelial cells hucec
Figure 1. Circ_0000228 was remarkably up-modulated in CC tissues and cell lines. (a) A volcano plot was employed to analyze the differentially expressed circRNAs in the GEO dataset (GSE113696) that were remarkably up- and down-modulated in CC cell lines, compared with normal cervical <t>epithelial</t> cells; (b) A heat maps was utilized to present the significantly highly expressed circRNAs in CC; (c) Circ_0000228 expression in 40 CC tissues/paired adjacent cervical tissues was examined by qRT-PCR; (d) Circ_0000228 expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (e) ZEB1 mRNA expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (f) Circ_0000228 expression in <t>HUCEC</t> and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (g) Circ_0000228 expression in the nucleus and cytoplasm of HCC94 and C33A cells was detected by qRT-PCR. All of the experiments were performed in triplicate. ***P < 0.001.
Human Normal Cervical Epithelial Cells Hucec, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+normal+cervical+epithelial+cells/pm34308761-41-11-21?v=ATCC
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human normal cervical epithelial cells (hncec)  (CH Instruments)
90
CH Instruments human normal cervical epithelial cells (hncec)
Figure 1. Circ_0000228 was remarkably up-modulated in CC tissues and cell lines. (a) A volcano plot was employed to analyze the differentially expressed circRNAs in the GEO dataset (GSE113696) that were remarkably up- and down-modulated in CC cell lines, compared with normal cervical <t>epithelial</t> cells; (b) A heat maps was utilized to present the significantly highly expressed circRNAs in CC; (c) Circ_0000228 expression in 40 CC tissues/paired adjacent cervical tissues was examined by qRT-PCR; (d) Circ_0000228 expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (e) ZEB1 mRNA expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (f) Circ_0000228 expression in <t>HUCEC</t> and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (g) Circ_0000228 expression in the nucleus and cytoplasm of HCC94 and C33A cells was detected by qRT-PCR. All of the experiments were performed in triplicate. ***P < 0.001.
Human Normal Cervical Epithelial Cells (Hncec), supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+normal+cervical+epithelial+cells/pmc09161891-60-1-22?v=CH+Instruments
Average 90 stars, based on 1 article reviews
human normal cervical epithelial cells (hncec) - by Bioz Stars, 2026-08
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normal cervical epithelial cells human uterus  (ScienCell)
90
ScienCell normal cervical epithelial cells human uterus
Figure 1. Circ_0000228 was remarkably up-modulated in CC tissues and cell lines. (a) A volcano plot was employed to analyze the differentially expressed circRNAs in the GEO dataset (GSE113696) that were remarkably up- and down-modulated in CC cell lines, compared with normal cervical <t>epithelial</t> cells; (b) A heat maps was utilized to present the significantly highly expressed circRNAs in CC; (c) Circ_0000228 expression in 40 CC tissues/paired adjacent cervical tissues was examined by qRT-PCR; (d) Circ_0000228 expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (e) ZEB1 mRNA expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (f) Circ_0000228 expression in <t>HUCEC</t> and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (g) Circ_0000228 expression in the nucleus and cytoplasm of HCC94 and C33A cells was detected by qRT-PCR. All of the experiments were performed in triplicate. ***P < 0.001.
Normal Cervical Epithelial Cells Human Uterus, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+normal+cervical+epithelial+cells/pmc05740563-92-6-11?v=ScienCell
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normal cervical epithelial cells human uterus - by Bioz Stars, 2026-08
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Image Search Results


Figure 1. Circ_0000228 was remarkably up-modulated in CC tissues and cell lines. (a) A volcano plot was employed to analyze the differentially expressed circRNAs in the GEO dataset (GSE113696) that were remarkably up- and down-modulated in CC cell lines, compared with normal cervical epithelial cells; (b) A heat maps was utilized to present the significantly highly expressed circRNAs in CC; (c) Circ_0000228 expression in 40 CC tissues/paired adjacent cervical tissues was examined by qRT-PCR; (d) Circ_0000228 expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (e) ZEB1 mRNA expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (f) Circ_0000228 expression in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (g) Circ_0000228 expression in the nucleus and cytoplasm of HCC94 and C33A cells was detected by qRT-PCR. All of the experiments were performed in triplicate. ***P < 0.001.

Journal: Bioengineered

Article Title: Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis.

doi: 10.1080/21655979.2021.1954846

Figure Lengend Snippet: Figure 1. Circ_0000228 was remarkably up-modulated in CC tissues and cell lines. (a) A volcano plot was employed to analyze the differentially expressed circRNAs in the GEO dataset (GSE113696) that were remarkably up- and down-modulated in CC cell lines, compared with normal cervical epithelial cells; (b) A heat maps was utilized to present the significantly highly expressed circRNAs in CC; (c) Circ_0000228 expression in 40 CC tissues/paired adjacent cervical tissues was examined by qRT-PCR; (d) Circ_0000228 expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (e) ZEB1 mRNA expression in 21 patients with stage I CC and 19 patients with stage II CC was analyzed by qRT-PCR, respectively; (f) Circ_0000228 expression in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (g) Circ_0000228 expression in the nucleus and cytoplasm of HCC94 and C33A cells was detected by qRT-PCR. All of the experiments were performed in triplicate. ***P < 0.001.

Article Snippet: Four human CC cell lines (HeLa, HCC94, SW756, and C33A) and human normal cervical epithelial cells (HUCEC) were procured from the American Type Culture Collection (ATCC, Bethesda, MD, USA) and the Chinese Academy of Sciences Committee Type Culture Collection Cell Bank (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Figure 3. Circ_0000228 modulated LOXL2 expression through miR-195-5p. (a) A volcano plot was adopted to show the miRNAs that were remarkably up- and down-modulated in CC tissues compared with normal cervical tissue in the GEO dataset (GSE86100); (b) The Venn diagram was used to screen out the differentially expressed miRNAs in GSE86100, which contained complementary binding sites with circ_0000228; (c) Bioinformatics analysis indicated that high LOXL2 expression suggested poor clinical outcome of CC patients. (d) The complementary binding sites of circ_0000228 and miR-195-5p, miR-195-5p and LOXL2 3UTR are presented, and the corresponding luciferase reporter vectors were designed; (E-F) Dual-luciferase reporter gene experiments confirmed that the binding sites between circ_0000228 and miR-195-5p, miR-195-5p and LOXL2 3UTR were functional. (g) MiR-195-5p expression in 40 pairs of CC tissues/adjacent cervical tissues was detected by qRT-PCR; (h) MiR-195-5p expression in stage I and stage II CC patients was detected by qRT-PCR, respectively; (I) MiR-195-5p in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR.(j) LOXL2 mRNA expression in 40 pairs of CC tissues/adjacent cervical tissues was detected by qRT-PCR; (k) LOXL2 mRNA expression in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (L) LOXL2 mRNA expression in stage 1 and stage 2 CC patients was detected by qRT-PCR; (m-o) Pearson’s correlation analysis of the correlation relationships among circ_0000228 miR-195-5p, and LOXL2 expression in CC tissues; (p) qRT- PCR was employed to detect miR-195-5p expression after co-transfection with circ_0000228 siRNA and miR-195-5pin in CC cell lines; (q) qRT-PCR and Western blot experiments were employed to detect LOXL2 expression after co-transfection with circ_0000228 siRNA and miR-195-5p inhibitors in CC cell lines; All of the experiments were performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001.

Journal: Bioengineered

Article Title: Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis.

doi: 10.1080/21655979.2021.1954846

Figure Lengend Snippet: Figure 3. Circ_0000228 modulated LOXL2 expression through miR-195-5p. (a) A volcano plot was adopted to show the miRNAs that were remarkably up- and down-modulated in CC tissues compared with normal cervical tissue in the GEO dataset (GSE86100); (b) The Venn diagram was used to screen out the differentially expressed miRNAs in GSE86100, which contained complementary binding sites with circ_0000228; (c) Bioinformatics analysis indicated that high LOXL2 expression suggested poor clinical outcome of CC patients. (d) The complementary binding sites of circ_0000228 and miR-195-5p, miR-195-5p and LOXL2 3UTR are presented, and the corresponding luciferase reporter vectors were designed; (E-F) Dual-luciferase reporter gene experiments confirmed that the binding sites between circ_0000228 and miR-195-5p, miR-195-5p and LOXL2 3UTR were functional. (g) MiR-195-5p expression in 40 pairs of CC tissues/adjacent cervical tissues was detected by qRT-PCR; (h) MiR-195-5p expression in stage I and stage II CC patients was detected by qRT-PCR, respectively; (I) MiR-195-5p in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR.(j) LOXL2 mRNA expression in 40 pairs of CC tissues/adjacent cervical tissues was detected by qRT-PCR; (k) LOXL2 mRNA expression in HUCEC and CC cell lines (HeLa, HCC94, SW756, C33A) was detected by qRT-PCR; (L) LOXL2 mRNA expression in stage 1 and stage 2 CC patients was detected by qRT-PCR; (m-o) Pearson’s correlation analysis of the correlation relationships among circ_0000228 miR-195-5p, and LOXL2 expression in CC tissues; (p) qRT- PCR was employed to detect miR-195-5p expression after co-transfection with circ_0000228 siRNA and miR-195-5pin in CC cell lines; (q) qRT-PCR and Western blot experiments were employed to detect LOXL2 expression after co-transfection with circ_0000228 siRNA and miR-195-5p inhibitors in CC cell lines; All of the experiments were performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001.

Article Snippet: Four human CC cell lines (HeLa, HCC94, SW756, and C33A) and human normal cervical epithelial cells (HUCEC) were procured from the American Type Culture Collection (ATCC, Bethesda, MD, USA) and the Chinese Academy of Sciences Committee Type Culture Collection Cell Bank (Shanghai, China).

Techniques: Expressing, Binding Assay, Luciferase, Functional Assay, Quantitative RT-PCR, Cotransfection, Western Blot